GLYCOLIC ACID OXIDASE

Activation of glycolic acid oxidase in plants.

The enzyme which oxidizes glycolic acid to glyoxylic acid is present in sufficient concentrations to support active respiration in a wide variety of plants (1). Glycolic acid itself is present in plants in small amounts (2), and, as yet, it has been the only 2-carbon organic acid which has been found to accumulate Cl4 during short time photosynthesis experiments with Cl402 (3). Thus both the fr...

Nature and Distribution of Glycolic Acid Oxidase in Plants.

Effect of anions on the catalytic activity of pig liver glycolic acid oxidase.

I. Pig liver glycolic acid oxidase (glycolate: 02 oxidoreductase, EC 1.1.3.1 ) can function with either 2,6-dichlorophenolindophenol (DCIP) (Vmax----125o moles of glycolate per mole of ravin per rain) or 02 (Vmax = 620 moles of glycolate per mole of ravin per rain)9,1° as the electron acceptor. 2. Straight-chain monocarboxylic acids are non-competitive inhibitors of this enzyme with either glyc...

Glycolic Acid Oxidase Activity in Cell-free Preparations of Blue-Green Algae.

Glycolic acid oxidase activity was detected in cell-free preparations of Anabaena flos-aquae and Oscillatoria sp. by the reduction of 2,6-dichlorophenolindophenol and by the formation of glyoxylate. Enzyme activity was localized in the 20,000 times gravity supernatant fraction, and optimal activity was obtained at pH 8.0. Activity was lost on storing the preparation at 4 C and could not be rest...

The role of glycolic acid oxidase in the respiration of leaves.

It has also been shown that glycolic acid oxidase, together with the pyridine nucleotide-linked glyoxylic acid reductase, catalyzes the oxidation of reduced pyridine nucleotides by molecular oxygen in model systems (2). In a previous report it was shown that bisulfite addition compounds of aldehydes are effective and highly specific competitive inhibitors of glycolic acid oxidase (8). These com...